Fungal Transcription Factor Database
Detalied information of reference
Pubmed ID (PMID)
12080340
Paper type
Title
A new family of plant transcription factors displays a novel ssDNA-binding surface.
Abstract
The crystal structure of p24, the single-stranded DNA (ssDNA) binding subunit of the plant defense transcription factor PBF-2, has been determined to 2.3 A resolution. p24 is representative of a novel family of ubiquitous plant-specific proteins that we refer to as the Whirly family because of their quaternary structure. PBF-2 is composed of four p24 molecules that interact through a helix-loop-helix motif. This interaction produces a central pore, with beta-strands radiating outwards, resulting in a whirligig appearance to the quaternary structure. The noncrystallographic C(4) symmetry arrangement of p24 subunits is novel for ssDNA binding proteins and may explain the binding specificity of PBF-2. This structural arrangement also supports the role of PBF-2 in binding melted promoter regions to modulate gene expression.
Authors
Desveaux D, Allard J, Brisson N, Sygusch J.
Affiliation
Department of Biochemistry, Université de Montréal, CP 6128, Station Centre-Ville, Montréal, Québec, H3C 3J7, Canada.
Journal / Pages
Nature structural biology., 2002 : 9(7), 512~517