Fungal Transcription Factor Database
Detalied information of reference
Pubmed ID (PMID)
8196641
Paper type
Title
The Drosophila tissue-specific factor Grainyhead contains novel DNA-binding and dimerization domains which are conserved in the human protein CP2.
Abstract
We have mapped the regions in the Drosophila melanogaster tissue-specific transcription factor Grainyhead that are required for DNA binding and dimerization. These functional domains correspond to regions conserved between Grainyhead and the vertebrate transcription factor CP2, which we show has similar activities. The identified DNA-binding domain is large (263 amino acids) but contains a smaller core that is able to interact with DNA at approximately 400-fold lower affinity. The major dimerization domain is located in a separate region of the protein and is required to stabilize the interactions with DNA. Our data also suggest that Grainyhead activity can be modulated by an N-terminal inhibitory domain.
Authors
Uv AE, Thompson CR, Bray SJ.
Affiliation
Department of Anatomy, University of Cambridge, England.
Journal / Pages
Molecular and cellular biology., 1994 : 14(6), 4020~4031